DISPLAYING IMAGES

Image data are displayed in XY windows, with XZ and YZ projections available for 3D images. The above example shows a 3D confocal image stack of a sub-resolution bead used to acquired empirical point-spread functions. The yellow guidelines can be moved around to re-slice the image volume with all views updated on-the-fly. Custom look-up tables can be applied to single channel data, and full support for brightness, contrast, gamma, floor and ceiling saturation level adjustment is provided for monochrome and RGB images to help you visualize your images. 4D images are handled as groups of 3D z-series. Tool palettes (below) allow you to navigate among images, select tools such as mover, zoom and area mean:

“Reslicing” XZ and YZ projections can be done at arbitrary angles to maintain structures of interest in view if they are rotated in the XY plane, as shown for the axon below stained with neurofilament antibody. The images on the left show XZ/YZ projections “sliced” at right angles; part of the fiber is missing at the top of the YZ projection. By tilting the yellow guidelines, you can angle the planes of projection so they are at right angles to the subject rather than the window (right):

PERFORMING CALCULATIONS ON IMAGES