ImageTrak is an image analysis program designed for converting, viewing, and quantitatively analyzing multidimensional scientific image data. Either single images, 3D image stacks or higher-dimensional data (3D vs. time, volumetric spectral datasets) are supported. Although ImageTrak was specifically designed for fluorescence microscopy, virtually any type of image data can be analyzed.

Features include:

1) sophisticated visualization and quantitative analysis of multidimensional image data, which may originate from microscopy, EM, MRI, scanned Western blots, etc… Ability to handle large (10’s of GB) images.
2) complex image math from user-defined formulas.
3) ability to analyze and perform automated statistics on a large number of ROIs individually
4) XZ/YZ projection views sliced at arbitrary tilt angles.
5) Cross-fade animations for examining co-localization in double or triple stained samples
6) Manual or auto-registration and export as animations of time series images
7) Advanced visualization, processing and analysis of spectral datasets.  Spectral image processing has been greatly expanded in the v4.3 and later releases. This includes Spectral Scatter Analysis, a method to quantitate multidimensional lambda stacks.
8) Imports TIFFs, raw binary, Nikon ics/ids and Zeiss czi files directly, also imports TIFF sets from Nikon Elements, Thor and Leica systems. Big TIFFs (.btf files) from Olympus slide scanners are also supported.

Software is Mac only and will run on macOS High Sierra or later. It has been updated to run natively under 64 bit and Intel x86 or Apple Silicon.

Terms:
ImageTrak is freeware and is supplied “as is”. The author cannot be responsible for any losses or damages arising from the use of this software. You are free to use and redistribute this software and documentation for non-commercial purposes, provided no modifications are made to the code or documentation. You may not charge any fee for the redistribution of the ImageTrak software or its documentation.
Please credit the author (Peter K. Stys) and cite the ImageTrak homepage (https://stysneurolab.org/imagetrak/) in all publications where you made use of this software.

Download:

To install, download and double-click the pkg installer (you may get a message like “Can’t load this file type“, just ignore it and continue with the download by pressing the Download button). All related files reside within the ImageTrak package. No other files are installed except an ImageTrak Preferences file is created at first launch. The latest Apple OS’s have made installation of non-App Store software very cumbersome. If your OS X security settings get in the way, go to System Settings > Privacy & Security. Near the bottom under Security you will see a message like “ITRKinstaller…pkg” was blocked to protect your Mac. Press the Open Anyway button, enter your admin password and proceed with the installation. The User’s Manual and various HTML help pages are integrated into the application. This is still rudimentary.

Here are some sample images so you can try out the software if you don’t have any images of your own handy.

Page and software installer last updated 2025-03-21.